Journal: ACS applied materials & interfaces

Article Title: Dual-Targeting Strategy to Repurpose Cetuximab with HFn Nanoconjugates for Immunotherapy of Triple-Negative Breast Cancer.

doi: 10.1021/acsami.5c06626

Figure Lengend Snippet: Figure 5. TfR1 and EGFR expression in U-87 MG and MDA-MB-231 cells. (A) Representative Western blot of total lysates of U-87 MG and MDA-MB-231 cells (15 μg) blotted as indicated. (B) Densitometric quantification of the TfR1 and EGFR signal normalized on the corresponding β-actin signal. Bar graphs show mean ± s.e. of 3 independent replicates. ** = p < 0.01 (two-tailed Student’s t-test). (C) Quantification of the fluorescence intensity of HFn alone or conjugated with CTX normalized by the number of nuclei. Data were obtained by measuring HFn−AF647 fluorescence in U-87 MG and MDA-MB-231 cells after 2 min of incubation with HFn or HFn−CTX. (D) Cell-surface EGFR and TfR1 levels as measured with CellProfiler and representative images of non-permeabilized cells stained for EGFR or TfR1. Nuclei were stained with Hoechst (blue), TfR1 and EGFR (red), and CFDA SE-labeled MDA-MB-231 cells (green). White asterisks mark the position of the MDA-MB-231 cells in the anti-TfR1 and anti-EGFR micrographs. Scale bar, 20 μm. Violin superplots show the mean ± s.e. of 3 independent replicates (1665 U-87 MG cells and 1065 MDA-MB-231 cells for EGFR; 2178 U-87 MG cells and 1751 MDA-MB-231 cells for TfR1). ** = p < 0.01; ****p < 0.0001 (unpaired two-tailed t-test).

Article Snippet: Primary antibodies: CD44 (clone IM7; Bio-Rad, MCA4703), CD31/PECAM-1 (Biotechne, AF3628), CD71 (clone D7G9X, Cell Signaling, 13113),32,33 EGFR (clone D38B1, Cell Signaling, 4267), βActin (clone D6A8, Cell Signaling, 8457), EGFR (clone 528, Merck, GR01), CD71 (clone MEM-75, Invitrogen, MA1-19137), and Ferritin Heavy Chain (clone EPR3005Y, Abcam, ab75972).

Techniques: Expressing, Western Blot, Two Tailed Test, Fluorescence, Incubation, Staining, Labeling

Journal: Animals : an open access journal from MDPI

Article Title: Antiviral Activity of 1-Deoxynojirimycin Extracts of Mulberry Leaves Against Porcine Epidemic Diarrhea Virus.

doi: 10.3390/ani15091207

Figure Lengend Snippet: Figure 2. DNJ inhibits PEDV infection in Vero-E6 cells. (A) The overall design of Vero E6 cells infected and treated with DNJ (25 µM, 50 µM, 100 µM). The green line indicates drug treatment, and the orange line indicates PEDV infection. (B) Samples were collected at 6, 12, and 24 h post-infection with SQ2014 (MOI = 0.1), and the protein levels of PEDV-N and actin were detected by Western blot. (C) After 12 h of infection, the protein samples with different concentrations of DNJ were detected by Western blot. (D) Quantitative detection of PEDV S and N mRNA level by qRT-PCR. (E) The cells were collected 12 h after infection, and the virus titer of SQ2014 was detected by PFU. The means and standard deviations of three independent experimental replicates are shown. ** p < 0.01; *** p < 0.001.

Article Snippet: The PEDV strain SQ2014 (GenBank accession No. KP728470) and Rabbit anti-PEDV N protein polyclonal antibodies were kindly provided by Professor Qian Yingjuan (Nanjing Agricultural University) [22]. βActin Rabbit polyclonal antibodies were purchased from Proteintech (20536-1-AP, Nanjing, China).

Techniques: Infection, Western Blot, Quantitative RT-PCR, Virus

Journal: Animals : an open access journal from MDPI

Article Title: Antiviral Activity of 1-Deoxynojirimycin Extracts of Mulberry Leaves Against Porcine Epidemic Diarrhea Virus.

doi: 10.3390/ani15091207

Figure Lengend Snippet: Figure 3. The inhibition stage of DNJ on PEDV. (A) This section describes the comprehensive approach for infecting Vero-E6 cells and administering DNJ at a concentration of 100 µM. The treatment procedure is divided into three separate phases: pre-treatment, co-treatment, and post- treatment. (B) In accordance with the treatment protocol depicted in (A), Western blot analysis was conducted to evaluate the protein levels of both the virus and actin. (C) DNJ directly targets PEDV SQ2014 experimental results.

Article Snippet: The PEDV strain SQ2014 (GenBank accession No. KP728470) and Rabbit anti-PEDV N protein polyclonal antibodies were kindly provided by Professor Qian Yingjuan (Nanjing Agricultural University) [22]. βActin Rabbit polyclonal antibodies were purchased from Proteintech (20536-1-AP, Nanjing, China).

Techniques: Inhibition, Concentration Assay, Western Blot, Virus